Composite

Part:BBa_K4015006:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-19)


Lcp-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The assembly is aimed to produce the lcp that can be secreted, while it has to notice that E.coli Bl21 itself does not have innate secretion system.


Source

HlyA and LcpVH2 are assembled by Gibson assembly, and the product is ligated into pET23a.

References